Effect of Temperature on Enzyme-catalyzed Reaction

Effect of Temperature on Enzyme-catalyzed ReactionYasmine DabashIntroductionTopic The denaturing of enzymes.Purpose To advertize investigate catalysts and enzymes as well as their reactions to temperature change.Problem How does temperature affect the rate of an enzyme-catalyzed reaction?ResearchSource 1 http//www.worthington-biochem.com/introbiochem/tempeffects.htmlInformation found Like the absolute majority of chemical substance reactions, the rate of an enzyme-catalyzed reaction raises as the temperature elevates. According to the source, A ten degree Centigrade rise in temperature will increase the activity of most enzymes by 50 to 100%.Many enzymes ar unfavorably affected by intense heat. Because most animal enzymes quickly become denatured over 40C, nearly all enzyme activity is accomplished under that temperature. After some time, enzymes neutralize at even reasonable temperatures. The recommended temperature to store enzymes is 5C or lower but even some enzymes deactivate when frozen.Source 2 http//classroom.synonym.com/effects-temperature-enzyme-activity-biology-6049.htmlInformation found Collisions between molecules increase as temperature increases. This is because of the increase in pep pill and kinetic force that is a result of increased temperature. With rapider velocities, there will be less time between molecule collisions. This results in supplementary molecules scope activation energy, which elevates the rate of the reactions. Since the molecules are also moving quicker, collisions between enzymes and substrates also increase.Identify VariablesIndependent Variable liver-colored temperatureDependent Variable The rate at which the liver rises to the surface of the hydrogen peroxide.Controlled Variables Environment temperature, liver origin (same individual of the same species), concentration of hydrogen peroxide solution, water composition, click subject size, filter musical composition weight and density, beaker material, forceps mate rial, Petri dish material, filter paper material, and pipette material.Hypothesis If enzymes are heated to 70C then the rate of enzyme-catalyzed reactions will fall make because the denaturing of enzymes occurs after 40C.The PlanProcedureStep 1 Put on an apron, gloves, and safety goggles. Also, make sure hair is tied up by from your face and out of the way of any experimental material.Step 2 Use forceps to place raw liver in an open Petri dish. Use a pipette to put a few drops of hydrogen peroxide solution on the liver. Observe any chemical or physical changes. (Caution Rinse off any hydrogen peroxide that comes in contact with skin, eyes, or clothing)Step 3 Cut of a 5 cm x 5 cm piece of filter paper and absorb it with a sample of liver.Step 4 Fill 50 a 50 mL beaker with 25 mL of 1% hydrogen peroxide solution using a graduated cylinder to measure.Step 5 Use forceps to drop the livered filter paper to the prat of the solution. Record using a timer how long the filter paper takes t o rise to the surface. Seek assistance if needed.Step 6 Now, heat 25 mL of water and a sample of liver in a 50 mL beaker to 70C with on a hot dwelling house. (Caution Hot plates are called hot plates for a reason their hot. They can burn you. Seek adult assistance if young or incapable.)Step 7 Turn the hot plate OFF and remove the beaker from the hot plate using beaker tongs. Then remove the liver from the water using forceps and set the sample into a Petri dish.Step 8 Repeat the previous experiment with the heated liver. Records results then analyze the data.Step 9 Compare and contrast the data sets from each part of the experiment.DataLiver temperatureThe time it took for the liver to reach the surface of a 1% hydrogen peroxide. (seconds)Room Temperature Liver5.73 seconds70C Liver7.22 secondsResults and UnderstandingsGraphingData analysisWhen analyzing the results of the experiment, it is slow to see that there are trends in the data. The relationship between enzyme activity and temperature is inversely proportional. The higher the temperature of the enzymes, the faster they react and the less time the livered filter paper takes to rise to the surface of a 1% hydrogen peroxide solution. However, after 40C, the denaturing of enzymes therefore slowing down enzyme-catalyzed reactions. This is because as the denaturing of enzymes occurs they no longer see to it their specific lock to which they are key to.Though there was non any lack in accuracy, there was an extreme lack of precision during this experiment. Firstly, the attendant scientific hands were not at all interested in the experiment making it exceedingly difficult to attempt to get them on task, sodding(a) the experiment, and get steps that require the cooperation of more than one individual done. It is also importance to recognize the limitation of time allotted for previously mentioned tasks to be use one class period. Also, it would have made all the difference in the world if the time it took for the filter paper to rise in increments of 5 mL could have been measured since it rose through 25 mL of 1% hydrogen peroxide solution. In doing this, it would be seen whether the filter paper accelerated positively or decelerated as it rose through the solution. But due to time constraints, lack of expertise, and lack of an extremely precise timing device, such an idea could not be executed. But even before trying to accomplish that out of the ballpark idea, it would have been better to allow more trials for each temperature of liver and perchance entertain the addition of higher or lower temperatures of liver. Perhaps refrigerated liver? Even better, liver at or around 40C. subtle how enzymes react to heat is vital for a number of biologists globally. Especially since biologists are required to properly handle and store enzymes. Enzymes are best stored below 5C in order for them to not o dormant. Also, it is important for real life biologists to understand that enzymes will wo rk faster in heat whole up to a certain point. For example, in many animals, enzymes denature after they hit 40C. Almost all enzyme activity happens below that temperature.Conclusion occupyIn correlation to the hypothesis, the outcome of the experiment proved the following hypothesis If enzymes are heated to 70C then the rate of enzyme-catalyzed reactions will minify because the denaturing of enzymes occurs after 40C. The hypothesis gathered from the evidence pertaining to the collision opening ended up being true not only on paper but in real life experimentation and scenarios as well. Since the chemical reaction slowed when the livered filter paper was heated to 70C, then it is easy to regularise with utmost certainty that the relationship between temperature and reaction time is indirectly proportional up until 40C.In regards to the purpose of the lab experiment, many investigations have been made that further our knowledge on how to properly handle enzymes temperature wise. Which temperature are too hot, which are not hot enough, etc. Also, from the information gathered, other skilful assumptions can be made on the proper storage of enzymes. Since it is known that enzymes, over time, become dormantwouldnt it be ideal to freeze them so they retain their activity? But perhaps, that is for yet another experiment.Evidence and ElaborationEvidence 1The collision theory states that as temperature rises, particles move more rigorously as collide more often. When these molecules bump into one another, reactions occurin this example, the enzyme-catalyzed reaction is the result. The amount of collisions it takes for a full reaction to occur is the activation energy of a chemical reaction. This theory is the first piece of evidence for the increase in enzyme-catalyst activity then temperature increases.Evidence 2The Induced Fit theory of enzymes accounts for the fact that the activity and substrate of an enzyme are complementary and mold to one anothers ice-cube tray, or as biologymad.com comparables to say, Mould to each other like hand in glove. The links in substrates and enzymes bend when enzyme-substrate compounds form, deteriorating them significantly. Unlike the Lock and Key theory of enzymes, the Induced Fit theory accounts for catalytic action and the products of chemical reactions pertaining to enzymes.Works CitedEnzymes. Biology Mad. Master Frameset, 07 Nov. 2005. Web. 07 Dec. 2014.Introduction to Enzymes Temperature Effects. Worthington Biochemistry. Worthington Biochemical Corporation, n.d. Web. 07 Dec. 2014.Santhosh, Lakshmi. The Effects of Temperature on Enzyme Activity. Synonym. Demand Media, 14 Aug. 2013. Web. 05 Dec. 2014.